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biotinylated vectorlabs ba 0500 5 bsa  (Vector Laboratories)


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    Vector Laboratories biotinylated vectorlabs ba 0500 5 bsa
    Biotinylated Vectorlabs Ba 0500 5 Bsa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+streptavidin+biotinylated+antibody/Biotinylated+Anti-Streptavidin+Antibody/pm41759513-561-216-217
    Average 96 stars, based on 1784 article reviews
    biotinylated vectorlabs ba 0500 5 bsa - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Hybridization:

    Article Title: BK Ca ( Slo ) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster
    Article Snippet: Each Affymetrix gene chip Drosophila array (Affymetrix, Santa Clara, CA, USA) was hybridized with the fragmented and biotin-labeled target (4.5 μg) in 200 μL of hybridization cocktail. .. Target denaturation was performed at 99 °C for 2 min and then 45 °C for 5 min, followed by hybridization for 18 h. Then the arrays were washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St. Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. The chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console Software.

    Article Title: An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes
    Article Snippet: RNA amplification and labeling was performed from 50 ng total RNA by the WT-Ovation Pico RNA amplification system (NuGen Technologies, Inc., San Carlos, CA, USA) as described previously [ ]. .. Each Affymetrix Rat Gene array (RaGene 1.0 ST v1) (Affymetrix, Santa Clara, CA, USA) was hybridized with fragmented and biotin-labeled target for 18 h. Arrays were then washed and stained using Gene-chip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console software.

    Article Title: Demyelinating strain of mouse hepatitis virus infection bridging innate and adaptive immune response in the induction of demyelination
    Article Snippet: For microarray analysis, total RNA was isolated from spinal cords of RSA59, RSMHV2 and mock-infected mice using the Qiagen RNeasy mini kit and DNase treatment. cDNA was synthesized using the Ovation Pico WTA system V2 (Nugen Technologies), fragmented, and labeled with biotin followed by hybridization with Affymetrix gene chip mouse gene 1.0 ST array that contains 750,000 unique 25mer oligonucleotide features for 28,000 gene level probe sets. .. Arrays were washed and stained using GeneChip fluidics station 450 and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories). ..

    Article Title: Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine coronavirus-induced neuroinflammation
    Article Snippet: .. Antibody-based amplification of hybridization signals was done using goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000 7G, using Command Console Software.

    Article Title: Adult zymosan re-exposure exacerbates the molecular alterations in the brainstem rostral ventromedial medulla of rats with early life zymosan-induced cystitis
    Article Snippet: .. The arrays were then washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000 using the Command Console Software.

    Article Title: RNA-seq in grain unveils fate of neo- and paleopolyploidization events in bread wheat ( Triticum aestivum L.)
    Article Snippet: Fragmentation of 15 μg of labeled cDNA was carried out for 35 minutes at 94°C, followed by hybridization for 16 hours at 45°C to Affymetrix wheat GeneChip ® oligonucleotide arrays. .. After hybridization, the arrays were washed with two different buffers (stringent: 6 SSPE, 0.01% Tween 20; and non-stringent: 100 mm MES, 0.1 M Na + , 0.01% Tween 20) and stained with a complex solution including Streptavidin R-Phycoerythrin conjugate (Molecular Probes, [ ]) and anti-streptavidin biotinylated antibody (Vector Laboratories, [ ]). .. The washing and staining steps were performed in a GeneChip ® Fluidics Station 450 (Affymetrix).

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Staining:

    Article Title: BK Ca ( Slo ) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster
    Article Snippet: Each Affymetrix gene chip Drosophila array (Affymetrix, Santa Clara, CA, USA) was hybridized with the fragmented and biotin-labeled target (4.5 μg) in 200 μL of hybridization cocktail. .. Target denaturation was performed at 99 °C for 2 min and then 45 °C for 5 min, followed by hybridization for 18 h. Then the arrays were washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St. Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. The chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console Software.

    Article Title: An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes
    Article Snippet: RNA amplification and labeling was performed from 50 ng total RNA by the WT-Ovation Pico RNA amplification system (NuGen Technologies, Inc., San Carlos, CA, USA) as described previously [ ]. .. Each Affymetrix Rat Gene array (RaGene 1.0 ST v1) (Affymetrix, Santa Clara, CA, USA) was hybridized with fragmented and biotin-labeled target for 18 h. Arrays were then washed and stained using Gene-chip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console software.

    Article Title: Demyelinating strain of mouse hepatitis virus infection bridging innate and adaptive immune response in the induction of demyelination
    Article Snippet: For microarray analysis, total RNA was isolated from spinal cords of RSA59, RSMHV2 and mock-infected mice using the Qiagen RNeasy mini kit and DNase treatment. cDNA was synthesized using the Ovation Pico WTA system V2 (Nugen Technologies), fragmented, and labeled with biotin followed by hybridization with Affymetrix gene chip mouse gene 1.0 ST array that contains 750,000 unique 25mer oligonucleotide features for 28,000 gene level probe sets. .. Arrays were washed and stained using GeneChip fluidics station 450 and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories). ..

    Article Title: Adult zymosan re-exposure exacerbates the molecular alterations in the brainstem rostral ventromedial medulla of rats with early life zymosan-induced cystitis
    Article Snippet: .. The arrays were then washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000 using the Command Console Software.

    Article Title: RNA-seq in grain unveils fate of neo- and paleopolyploidization events in bread wheat ( Triticum aestivum L.)
    Article Snippet: Fragmentation of 15 μg of labeled cDNA was carried out for 35 minutes at 94°C, followed by hybridization for 16 hours at 45°C to Affymetrix wheat GeneChip ® oligonucleotide arrays. .. After hybridization, the arrays were washed with two different buffers (stringent: 6 SSPE, 0.01% Tween 20; and non-stringent: 100 mm MES, 0.1 M Na + , 0.01% Tween 20) and stained with a complex solution including Streptavidin R-Phycoerythrin conjugate (Molecular Probes, [ ]) and anti-streptavidin biotinylated antibody (Vector Laboratories, [ ]). .. The washing and staining steps were performed in a GeneChip ® Fluidics Station 450 (Affymetrix).

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Amplification:

    Article Title: BK Ca ( Slo ) Channel Regulates Mitochondrial Function and Lifespan in Drosophila melanogaster
    Article Snippet: Each Affymetrix gene chip Drosophila array (Affymetrix, Santa Clara, CA, USA) was hybridized with the fragmented and biotin-labeled target (4.5 μg) in 200 μL of hybridization cocktail. .. Target denaturation was performed at 99 °C for 2 min and then 45 °C for 5 min, followed by hybridization for 18 h. Then the arrays were washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St. Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. The chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console Software.

    Article Title: An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes
    Article Snippet: RNA amplification and labeling was performed from 50 ng total RNA by the WT-Ovation Pico RNA amplification system (NuGen Technologies, Inc., San Carlos, CA, USA) as described previously [ ]. .. Each Affymetrix Rat Gene array (RaGene 1.0 ST v1) (Affymetrix, Santa Clara, CA, USA) was hybridized with fragmented and biotin-labeled target for 18 h. Arrays were then washed and stained using Gene-chip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich, St Louis, MO, USA) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000, using Command Console software.

    Article Title: Demyelinating strain of mouse hepatitis virus infection bridging innate and adaptive immune response in the induction of demyelination
    Article Snippet: For microarray analysis, total RNA was isolated from spinal cords of RSA59, RSMHV2 and mock-infected mice using the Qiagen RNeasy mini kit and DNase treatment. cDNA was synthesized using the Ovation Pico WTA system V2 (Nugen Technologies), fragmented, and labeled with biotin followed by hybridization with Affymetrix gene chip mouse gene 1.0 ST array that contains 750,000 unique 25mer oligonucleotide features for 28,000 gene level probe sets. .. Arrays were washed and stained using GeneChip fluidics station 450 and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories). ..

    Article Title: Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine coronavirus-induced neuroinflammation
    Article Snippet: .. Antibody-based amplification of hybridization signals was done using goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000 7G, using Command Console Software.

    Article Title: Adult zymosan re-exposure exacerbates the molecular alterations in the brainstem rostral ventromedial medulla of rats with early life zymosan-induced cystitis
    Article Snippet: .. The arrays were then washed and stained using GeneChip Fluidic Station 450, and hybridization signals were amplified using antibody amplification with goat IgG (Sigma-Aldrich) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA, USA). .. Chips were scanned on an Affymetrix Gene Chip Scanner 3000 using the Command Console Software.

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).

    Software:

    Article Title: Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro
    Article Snippet: The labeled cRNA was fragmented by heat and ion-mediated hydrolysis and was hybridized to the Human Genome HU133A oligonucleotide array GeneChip (Affymetrix) which contains ∼500,000 spots comprised of 22,283 different probe sets representing 14,397 unique genes. .. The arrays were washed and stained using a GeneChip Fluidic Station 450 (Affymetrix), and hybridization signals were amplified utilizing antibody amplification with goat IgG (Sigma-Aldrich, Saint Louis, MO) and anti-streptavidin biotinylated antibody (Vector Laboratories, Burlingame, CA) and then were scanned using an Affymetrix GeneChip Scanner 3000, using GeneChip Operating Software (GCOS) version 3.0. .. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software (Agilent Technologies, Santa Clara, CA).



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